Journal: Nucleic Acids Research
Article Title: Naphthalene diimide-naphthalimide dyads promote telomere damage by selectively targeting multimeric G-quadruplexes
doi: 10.1093/nar/gkaf301
Figure Lengend Snippet: Antitumoral efficacy of NDI-NIs in advanced 3D models. Advanced 3D spheroids were established from human colorectal cancer cells (HCT116) by growing the cells within drops of a methylcellulose scaffold matrix (day 0). After 2 days of culture (day 2), the spheres were treated with the indicated compound at final concentrations of 1, 2, and 5 μM. ( A ) Time course analysis of tumor spheroids growth, starting from 24 h after treatment (day 3), monitored by Incucyte ® S3 Live-Cell Analysis System (Essen BioScience, Ann Arbor, MI), 10× magnification. The results were expressed as the percentage of the spheroids area upon treatment relative to their own area before the compound administration. The histogram represents the mean values ± S.E.M. of at least four spheres. ( B ) Representative images of tumor spheroids growth described in panel (A). ( C ) Histological and immunohistochemical analysis of the spheroids generated from HCT116 cell lines and grown as in panel (A). Upper panels , representative images of immunostained sections. Lower panels, quantification of Ki-67, cleaved-caspase and γH2AX levels, expressed as percentage of positive cells. Thirty fields for condition were analyzed. The histograms represent the mean values ± S.D. of three independent experiments. * P < .05, ** P < .01, *** P < .001, and **** P < .0001.
Article Snippet: HCT116 TP53 -/- were obtained by Dr Vogelstein, Johns Hopkins University, and HCT116 BRCA2 -/- were kindly gifted from Dr Madalena Tarsounas.
Techniques: Cell Analysis, Immunohistochemical staining, Generated
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